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Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening

Received: 7 April 2015     Accepted: 11 April 2015     Published: 23 April 2015
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Abstract

The screening method for discovering new lipid synthesis inhibitors has not been well developed. Lipid synthesis genes are responsible for the synthesis of fatty acids in normal and tumor cells. Mortierella alpina(M. alpina) fungus has been found to produce large amounts of fatty acids because it possesses a full complement of lipid synthesis genes. We therefore hypothesized that M. alpina could be a good screening tool to find new compounds that inhibit fatty acid synthesis. We developed a M. alpina liquid culture based method to analyze the inhibitory effects of lipid synthesis inhibitors. We applied a color indicator method to monitor the oil production using some well documented lipid inhibitors (C75, Cerulenin) to verify the system, and analyzing pictures using image analysis software. The experimental drug nocodazole inhibited the lipid production in M. alpina almost to the same extent as the control inhibitors. Compared with biomass and protein levels, there was a profound effect on fatty acid level. We report the development of a fast and effective method for screening lipid synthesis inhibitors which can be used against obesity and cancer. This method can further be used to screen additional lipid and fatty acid synthesis inhibitors from natural compound libraries.

Published in Advances in Bioscience and Bioengineering (Volume 3, Issue 1)
DOI 10.11648/j.abb.20150301.11
Page(s) 1-10
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Fatty Acid Synthase, Image Analysis Software, Lipid Synthesis Inhibitor, Mortierella alpina, Nocodazole, Screening System

References
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Cite This Article
  • APA Style

    Md. Shofiul Azam, Zhennan Gu, Haiqin Chen, Yong Q. Chen. (2015). Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening. Advances in Bioscience and Bioengineering, 3(1), 1-10. https://doi.org/10.11648/j.abb.20150301.11

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    ACS Style

    Md. Shofiul Azam; Zhennan Gu; Haiqin Chen; Yong Q. Chen. Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening. Adv. BioSci. Bioeng. 2015, 3(1), 1-10. doi: 10.11648/j.abb.20150301.11

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    AMA Style

    Md. Shofiul Azam, Zhennan Gu, Haiqin Chen, Yong Q. Chen. Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening. Adv BioSci Bioeng. 2015;3(1):1-10. doi: 10.11648/j.abb.20150301.11

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  • @article{10.11648/j.abb.20150301.11,
      author = {Md. Shofiul Azam and Zhennan Gu and Haiqin Chen and Yong Q. Chen},
      title = {Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening},
      journal = {Advances in Bioscience and Bioengineering},
      volume = {3},
      number = {1},
      pages = {1-10},
      doi = {10.11648/j.abb.20150301.11},
      url = {https://doi.org/10.11648/j.abb.20150301.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20150301.11},
      abstract = {The screening method for discovering new lipid synthesis inhibitors has not been well developed. Lipid synthesis genes are responsible for the synthesis of fatty acids in normal and tumor cells. Mortierella alpina(M. alpina) fungus has been found to produce large amounts of fatty acids because it possesses a full complement of lipid synthesis genes. We therefore hypothesized that M. alpina could be a good screening tool to find new compounds that inhibit fatty acid synthesis. We developed a M. alpina liquid culture based method to analyze the inhibitory effects of lipid synthesis inhibitors. We applied a color indicator method to monitor the oil production using some well documented lipid inhibitors (C75, Cerulenin) to verify the system, and analyzing pictures using image analysis software. The experimental drug nocodazole inhibited the lipid production in M. alpina almost to the same extent as the control inhibitors. Compared with biomass and protein levels, there was a profound effect on fatty acid level. We report the development of a fast and effective method for screening lipid synthesis inhibitors which can be used against obesity and cancer. This method can further be used to screen additional lipid and fatty acid synthesis inhibitors from natural compound libraries.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening
    AU  - Md. Shofiul Azam
    AU  - Zhennan Gu
    AU  - Haiqin Chen
    AU  - Yong Q. Chen
    Y1  - 2015/04/23
    PY  - 2015
    N1  - https://doi.org/10.11648/j.abb.20150301.11
    DO  - 10.11648/j.abb.20150301.11
    T2  - Advances in Bioscience and Bioengineering
    JF  - Advances in Bioscience and Bioengineering
    JO  - Advances in Bioscience and Bioengineering
    SP  - 1
    EP  - 10
    PB  - Science Publishing Group
    SN  - 2330-4162
    UR  - https://doi.org/10.11648/j.abb.20150301.11
    AB  - The screening method for discovering new lipid synthesis inhibitors has not been well developed. Lipid synthesis genes are responsible for the synthesis of fatty acids in normal and tumor cells. Mortierella alpina(M. alpina) fungus has been found to produce large amounts of fatty acids because it possesses a full complement of lipid synthesis genes. We therefore hypothesized that M. alpina could be a good screening tool to find new compounds that inhibit fatty acid synthesis. We developed a M. alpina liquid culture based method to analyze the inhibitory effects of lipid synthesis inhibitors. We applied a color indicator method to monitor the oil production using some well documented lipid inhibitors (C75, Cerulenin) to verify the system, and analyzing pictures using image analysis software. The experimental drug nocodazole inhibited the lipid production in M. alpina almost to the same extent as the control inhibitors. Compared with biomass and protein levels, there was a profound effect on fatty acid level. We report the development of a fast and effective method for screening lipid synthesis inhibitors which can be used against obesity and cancer. This method can further be used to screen additional lipid and fatty acid synthesis inhibitors from natural compound libraries.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China

  • State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China

  • State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China

  • State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China

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