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Biocontrol of Mycosphaerella fijiensis Morelet, the Causal Agent of Black Sigatoka of Banana Tree (Musa spp.) Using Biopesticides in Côte d’Ivoire

Received: 1 October 2021     Accepted: 23 November 2021     Published: 29 November 2021
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Abstract

Black Leaf Streak Disease (BLSD) is the most restricting leaf disease to banana tree cultivation around the world. In order to control this disease, synthetic fungicides are extensively used. However, these products pose a real danger to environmental pollution and the health of applicators and consumers. Faced with this situation, alternative solutions must be considered to overcome their systematic use. This study was initiated in this context so as to assess the effectiveness of 20 biopesticide formulations on Mycosphaerella fijiensis conidia stemming from banana tree leaf samples originating from village plantations and showing the typical symptoms of stage 2 or 3 black Sigatoka. The assessment method used was that of dispersion in solid medium. Observations were made under an optical microscope equipped with a camera and consisted in determining the inhibition rates of conidia germ tube growth. A pathogenicity test was performed with 8 Mycosphaerella spp. isolates according to an inoculation technique under controlled conditions on whole plants of 5 banana tree cultivar vivoplants. The assessment of biopesticide protection effectiveness against BLSD was conducted on cultivar "Orishele" (very susceptible) with the most aggressive and virulent strain selected during isolate pathogenicity test. The results obtained show that all biopesticide formulations have significant antifungal activity on M. fijiensis conidia germ tube elongation. The average inhibition rate ranged from 83.31 to 99.89% for all biopesticides. The 8 M. fijiensis isolates used have all raised symptoms characteristic of black leaf streak disease regardless of the cultivar. In contrast, no isolate caused symptoms characteristic of Sigatoka disease (Yellow Sigatoka). Biopesticides and synthetic fungicides significantly reduced disease development rate compared to inoculated and untreated controls, but at varying degrees. Preventive treatment of seedlings is found to be much more effective than curative treatment. However, in order to protect banana and plantain tree varieties against M. fijiensis, both types of treatments are necessary.

Published in Advances in Bioscience and Bioengineering (Volume 9, Issue 4)
DOI 10.11648/j.abb.20210904.13
Page(s) 111-123
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Banana Trees, Mycosphaerella spp., Biopesticides, Inoculation; Preventive, Curative

References
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Cite This Article
  • APA Style

    Tuo Seydou, Kassi Koffi Fernand Jean-Martial, Camara Brahima, Ouédraogo Somgnogdin Léonard, Koné Daouda. (2021). Biocontrol of Mycosphaerella fijiensis Morelet, the Causal Agent of Black Sigatoka of Banana Tree (Musa spp.) Using Biopesticides in Côte d’Ivoire. Advances in Bioscience and Bioengineering, 9(4), 111-123. https://doi.org/10.11648/j.abb.20210904.13

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    ACS Style

    Tuo Seydou; Kassi Koffi Fernand Jean-Martial; Camara Brahima; Ouédraogo Somgnogdin Léonard; Koné Daouda. Biocontrol of Mycosphaerella fijiensis Morelet, the Causal Agent of Black Sigatoka of Banana Tree (Musa spp.) Using Biopesticides in Côte d’Ivoire. Adv. BioSci. Bioeng. 2021, 9(4), 111-123. doi: 10.11648/j.abb.20210904.13

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    AMA Style

    Tuo Seydou, Kassi Koffi Fernand Jean-Martial, Camara Brahima, Ouédraogo Somgnogdin Léonard, Koné Daouda. Biocontrol of Mycosphaerella fijiensis Morelet, the Causal Agent of Black Sigatoka of Banana Tree (Musa spp.) Using Biopesticides in Côte d’Ivoire. Adv BioSci Bioeng. 2021;9(4):111-123. doi: 10.11648/j.abb.20210904.13

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  • @article{10.11648/j.abb.20210904.13,
      author = {Tuo Seydou and Kassi Koffi Fernand Jean-Martial and Camara Brahima and Ouédraogo Somgnogdin Léonard and Koné Daouda},
      title = {Biocontrol of Mycosphaerella fijiensis Morelet, the Causal Agent of Black Sigatoka of Banana Tree (Musa spp.) Using Biopesticides in Côte d’Ivoire},
      journal = {Advances in Bioscience and Bioengineering},
      volume = {9},
      number = {4},
      pages = {111-123},
      doi = {10.11648/j.abb.20210904.13},
      url = {https://doi.org/10.11648/j.abb.20210904.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20210904.13},
      abstract = {Black Leaf Streak Disease (BLSD) is the most restricting leaf disease to banana tree cultivation around the world. In order to control this disease, synthetic fungicides are extensively used. However, these products pose a real danger to environmental pollution and the health of applicators and consumers. Faced with this situation, alternative solutions must be considered to overcome their systematic use. This study was initiated in this context so as to assess the effectiveness of 20 biopesticide formulations on Mycosphaerella fijiensis conidia stemming from banana tree leaf samples originating from village plantations and showing the typical symptoms of stage 2 or 3 black Sigatoka. The assessment method used was that of dispersion in solid medium. Observations were made under an optical microscope equipped with a camera and consisted in determining the inhibition rates of conidia germ tube growth. A pathogenicity test was performed with 8 Mycosphaerella spp. isolates according to an inoculation technique under controlled conditions on whole plants of 5 banana tree cultivar vivoplants. The assessment of biopesticide protection effectiveness against BLSD was conducted on cultivar "Orishele" (very susceptible) with the most aggressive and virulent strain selected during isolate pathogenicity test. The results obtained show that all biopesticide formulations have significant antifungal activity on M. fijiensis conidia germ tube elongation. The average inhibition rate ranged from 83.31 to 99.89% for all biopesticides. The 8 M. fijiensis isolates used have all raised symptoms characteristic of black leaf streak disease regardless of the cultivar. In contrast, no isolate caused symptoms characteristic of Sigatoka disease (Yellow Sigatoka). Biopesticides and synthetic fungicides significantly reduced disease development rate compared to inoculated and untreated controls, but at varying degrees. Preventive treatment of seedlings is found to be much more effective than curative treatment. However, in order to protect banana and plantain tree varieties against M. fijiensis, both types of treatments are necessary.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Biocontrol of Mycosphaerella fijiensis Morelet, the Causal Agent of Black Sigatoka of Banana Tree (Musa spp.) Using Biopesticides in Côte d’Ivoire
    AU  - Tuo Seydou
    AU  - Kassi Koffi Fernand Jean-Martial
    AU  - Camara Brahima
    AU  - Ouédraogo Somgnogdin Léonard
    AU  - Koné Daouda
    Y1  - 2021/11/29
    PY  - 2021
    N1  - https://doi.org/10.11648/j.abb.20210904.13
    DO  - 10.11648/j.abb.20210904.13
    T2  - Advances in Bioscience and Bioengineering
    JF  - Advances in Bioscience and Bioengineering
    JO  - Advances in Bioscience and Bioengineering
    SP  - 111
    EP  - 123
    PB  - Science Publishing Group
    SN  - 2330-4162
    UR  - https://doi.org/10.11648/j.abb.20210904.13
    AB  - Black Leaf Streak Disease (BLSD) is the most restricting leaf disease to banana tree cultivation around the world. In order to control this disease, synthetic fungicides are extensively used. However, these products pose a real danger to environmental pollution and the health of applicators and consumers. Faced with this situation, alternative solutions must be considered to overcome their systematic use. This study was initiated in this context so as to assess the effectiveness of 20 biopesticide formulations on Mycosphaerella fijiensis conidia stemming from banana tree leaf samples originating from village plantations and showing the typical symptoms of stage 2 or 3 black Sigatoka. The assessment method used was that of dispersion in solid medium. Observations were made under an optical microscope equipped with a camera and consisted in determining the inhibition rates of conidia germ tube growth. A pathogenicity test was performed with 8 Mycosphaerella spp. isolates according to an inoculation technique under controlled conditions on whole plants of 5 banana tree cultivar vivoplants. The assessment of biopesticide protection effectiveness against BLSD was conducted on cultivar "Orishele" (very susceptible) with the most aggressive and virulent strain selected during isolate pathogenicity test. The results obtained show that all biopesticide formulations have significant antifungal activity on M. fijiensis conidia germ tube elongation. The average inhibition rate ranged from 83.31 to 99.89% for all biopesticides. The 8 M. fijiensis isolates used have all raised symptoms characteristic of black leaf streak disease regardless of the cultivar. In contrast, no isolate caused symptoms characteristic of Sigatoka disease (Yellow Sigatoka). Biopesticides and synthetic fungicides significantly reduced disease development rate compared to inoculated and untreated controls, but at varying degrees. Preventive treatment of seedlings is found to be much more effective than curative treatment. However, in order to protect banana and plantain tree varieties against M. fijiensis, both types of treatments are necessary.
    VL  - 9
    IS  - 4
    ER  - 

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Author Information
  • Laboratory of Biotechnology, Agriculture and Valorisation of Biological Resources, UPR of Plant Physiology and Pathology, UFR Biosciences, University Félix Houphou?t-Boigny, Abidjan, C?te d'Ivoire

  • Laboratory of Biotechnology, Agriculture and Valorisation of Biological Resources, UPR of Plant Physiology and Pathology, UFR Biosciences, University Félix Houphou?t-Boigny, Abidjan, C?te d'Ivoire

  • Laboratory of Biotechnology, Agriculture and Valorisation of Biological Resources, UPR of Plant Physiology and Pathology, UFR Biosciences, University Félix Houphou?t-Boigny, Abidjan, C?te d'Ivoire

  • Institute of Environment and Agricultural Research (INERA), Bobo Dioulasso, Burkina Faso

  • Laboratory of Biotechnology, Agriculture and Valorisation of Biological Resources, UPR of Plant Physiology and Pathology, UFR Biosciences, University Félix Houphou?t-Boigny, Abidjan, C?te d'Ivoire

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