Research Article | | Peer-Reviewed

Effect of Fungicides and Application Methods for the Management of Pepper Wilt Complex Diseases in Ethiopia

Received: 29 November 2024     Accepted: 11 December 2024     Published: 27 December 2024
Views:       Downloads:
Abstract

Pepper Wilt complex disease is one of the devastating soil-borne diseases, due to which 68-70% yield loss is recorded. Effects of different fungicides and application methods on wilt incidence and yield of pepper (Capsicum annuum L.) were tested for two years at Fogera and for one year at Dera districts of South Gondar zone Ethiopia. Effects of fungicides viz. copper oxychloride (Isacope 50WP), mancozeb + metalaxyl (Omaxim), thiamethoxam + metalaxyl + difenoconazole (Apron Star 42WS), copper hydroxide (Sinoko) and application methods viz. seed +seedling treatment, seed + seedling treatment followed by spraying at crown region of the plant on wilt disease incidence and pepper yield were studied at Fogera and Dera districts of South Gondar zone Ethiopia. The fungicides were applied at the rates of 3 kg/ha, 2 kg/ha, 20 g/ha and 2.5 kg/ha for Isacope 50WP, Omaxim, Apron Star 42WS and Sinoko, respectively. The seeds of pepper treated with fungicides and untreated were sown in seedling bed. The 45-day old seedlings were uprooted from the seed bed and subjected to the seedling treatment in different fungicides for 30 minutes and were planted in field. The spraying of the fungicides at crown region of the plant was done at 15 days interval after the initiation of wilt disease. The data on wilt incidence and yield were recorded in each treatment. The price of pepper was assessed from the local market and the total price of the yield obtained from each treatment was computed on hectare basis. Input costs like fungicide and labor were converted into hectare basis according to their frequencies used. Based on the obtained data from the above-mentioned parameters economic analysis was performed. The result of the experiment indicates that, during 2020 year, the highest disease reduction (61.11% and 51.39%) over check and maximum dry pod yield (21.95 q/ha and 48.46 q/ha) was observed with T6 (seed treatment+ seedling treatment +foliar spraying with fungicide Omaxim (mancozeb + metalaxyl) at Fogera and Dera, respectively. Similarly, highest disease reduction (26.66%) and maximum green pod yield was observed in T6 at Fogera during 2021 year. Moreover, economic analysis revealed that T6 (application of seed treatment+ seedling treatment + foliar spraying of fungicide Omaxim within 15 days interval) is effective treatment.

Published in Advances in Bioscience and Bioengineering (Volume 12, Issue 4)
DOI 10.11648/j.abb.20241204.13
Page(s) 93-97
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Pepper Wilt, Economic Analysis, Fungicide, Seedling Treatment

1. Introduction
Pepper (Capsicum annum L.) is the most important vegetable crop in different parts of the world . It is important source of income to smallholder farmers and contributes to export earnings in Ethiopia . But its production is declining due to several factors including diseases . Among the known pepper diseases, wilt is major problem causing losses up to 70% . The disease has been observed to be caused by Fusarium spp., Phytophthora capsici, Rhizoctonia solani, Sclerotium rolfsii, and Verticillium spp. . Now days, crop failure due to wilt diseases has been common in Fogera plane and farmers are forced to abandon their production.
To minimize the problem, number of fungicides has been evaluated by different researchers. Among the fungicides that controlled wilt pathogens: carbendazim, and Ridomil against F. oxysporum, Ridomil against P. capsici, Carboxin and carbendazim against R. solani have been reported . Moreover, different methods of fungicide application have been evaluated for management of pepper wilt complex. Among them: seed and seedling treatment in combinations with foliar spraying was found to be more effective than seed and seedling treatment alone . However, there is a lack of information regarding the effectiveness of fungicides and application methods for wilt complex disease in Fogera plains. Therefore, the objective of the study was to evaluate fungicides and application methods for the management of pepper wilt complex.
2. Materials and Methods
The experiments were conducted at Fogera and Dera districts of South Gondar zone Ethiopia during 2020 and 2021 cropping seasons at Fogera and in 2020 at Dera district. Effects of fungicides viz. copper oxychloride (Isacope 50WP), mancozeb + metalaxyl (Omaxim), thiamethoxam + metalaxyl + difenoconazole (Apron Star 42WS), copper hydroxide (Sinoko) and application methods viz. seed +seedling treatment, seed + seedling treatment followed by spraying at crown region of the plant against wilt complex disease were studied. The fungicides were applied at the rates of 3 kg/ha, 2 kg/ha, 20 g/ha and 2.5 kg/ha for Isacope 50WP, Omaxim, Apron Star 42WS and Sinoko, respectively.
The seeds of pepper treated with fungicides and untreated were sown in seedling bed. The 45 day old seedlings were uprooted from the seed bed and subjected to the seedling treatment in different fungicides for 30 minutes and were planted in field. The spraying of the fungicides at crown region of the plant was done at 15 days interval after the initiation of wilt disease. The data on wilt incidence and yield were recorded in each treatment.
The price of dry and green pepper fruit yield was assessed from the local market and the total price of the yield obtained from each treatment was computed on hectare basis. Input costs like fungicide and labor were converted into hectare basis according to their frequencies used. Fungicides cost was estimated based on the price of the local market. Cost of the labor was in Birr per man-days; cost of spray and spray equipment to spray per hectare were also calculated.
Based on the obtained data from the above-mentioned parameters economic analysis was performed according to the procedure . The dominance analysis procedure as detailed was used to select potentially profitable treatments from the range that was tested and serve to eliminate some of the treatments from further consideration and thereby simplify the analysis. The dominant or dominated treatments were ranked from lowest to highest costs that vary (Tables 2 and 3). A dominated treatment is any treatment that has net benefits that are less than those of a treatment with lower costs that vary .
3. Result and Discussion
3.1. Effect of Fungicides and Application Methods on Pepper Wilt Incidence
Result revealed that some the fungicides and application methods significantly reduced wilt incidence and increased yield as compared to check (Table 1). The highest disease reduction (66.66%) over check was observed with T7 followed by T6 and T11 (each 61.11%) at Fogera during 2020 year. At Dera, highest disease reduction (each 51.39%) over check was observed with T6 and 11 followed by T9 (11.95%) and T10 (12.5%) during 2020 year. However, the highest plant wilt, greater than check, was observed in all T5, T8 and T12 which are seedling treated with Apron star at both locations during 2020 year. It might be due to toxicity of seed treatment recommended dose fungicide Apron star for seedling treatment. The fungicide had recommendation rate for seed treatment, but not had seedling treatment recommendation. The highest disease reduction over check was observed with T6 (21.1%), T7 (26.66%), T8 (38.88%), T9 (28.88%) and T12 (37.77%) at Fogera in 2021.
Superiority of T6 (Seed treatment + Seedling treatment + foliar spraying of fungicide omaxim) was confirmed as maximum dry pod yield of 48.46 q/ha and 21.95 q /ha at Dera and Fogera, respectively, in 2020 (Tables 1 and 2). Similarly, highest green pod yield of 107.22 q/ha was recorded on this treatment at Fogera in 2021 (Table 1). The present findings are supported by earlier works where in Seed treatment and seedling treatment when fungicides were sprayed at crown region of the plant, wilt incidence was significantly reduced. Seed treatment+ seedling treatment + spraying of Carbendazim + Metalaxyl proved most effective and recorded 59.8% disease reduction over check under field conditions . Seed treatment with either mefenoxam or metalaxyl is a viable alternative for effective control of P. capsici during seed germination, seedling emergence, and early growth stages . Other four fungicides tegula (tebuconazole), thiophanate methyl, ridomil gold (metalaxyl+mancozeb) and mancozeb significantly inhibits the radial growth of S. rolfsii under in vitro condition. Besides it, two fungicides thiophanate methyl and mancozeb substantially control the growth of S. rolfsii under in vivo condition responsible for causing collar rot disease in Chickpea .
Table 1. Effect of fungicides and application methods on wilt incidence and yield in pepper at Fogera during 2020 and 2021.

2020

2021

Tr. No.

Treatments

WI (%)

DR (%)

DPY (q/ha)

WI (%)

DR (%)

GPY (q/ha)

Seed Treatment + Seedling treatment

T1

Check

94.44

-

1.45

58.88

0

63.89

T2

Sinoko (CH)

61.11

33.33

7.38

47.78

11.1

63.61

T3

Omaxim (O)

55.56

38.88

8.16

62.22

-3.34

33.057

T4

Copper oxychloride (CO)

52.78

41.66

9.38

70

-11.12

28.61

T5

Apron star (A)

100

-5.56

0

50.55

8.33

72.5

Seed treatment + Seedling treatment + Foliar spraying

T6

O

33.33

61.11

21.95

37.78

21.1

107.22

T7

A+O+O

27.78

66.66

17.2

32.22

26.66

75.557

T8

T5+O

97.22

-2.78

0.13

20

38.88

90.557

T9

CH

69.44

25

7.96

30

28.88

86.943

T10

CO

55.56

38.88

8.99

63.33

-4.45

34.443

T11

A+CO+CO

33.33

61.11

12.18

75.56

-16.68

38.33

T12

T5+CO

100

-5.56

0

21.11

37.77

93.89

CV (%)

27.34

34.44

21.64

20.4

3.2. Economic Analysis of Fungicides and Application Methods Effects on Pepper Wilt
Economic analysis results showed that highest marginal rate of return was obtained from treatment three in 2020 at both Fogera and Dera and treatment four in 2021 at Fogera (Table 2 and 3). According to the manual for economic analysis, the recommendation is not necessarily based on the treatment with the highest marginal rate of return, the treatment with the highest net benefit, and nor the treatment with the highest yield. The identification of a recommendation is based on the minimum acceptable marginal rate of return, and the treatment with the highest net benefit together with an acceptable MRR becomes the tentative recommendation . In this study, 100% was considered as minimum acceptable rate of return for farmers’ recommendation. It is important to note that the acceptable minimum rate of return for farmers’ recommendation is 50 to 100% . Accordingly, the study revealed that T6 (application of seed treatment+ seedling treatment + foliar spraying of fungicide Omaxim within 15 days interval) is effective treatment.
Table 2. Economic analysis of fungicides and application methods effects on pepper wilt in 2020 and 2021 at Fogera.

2020

2021

Tr. No.

Treatments

TVC

NB

MRR

NB

MRR

Seed Treatment + Seedling treatment

T1

check

0

26100

230004

T2

Sinoko (CH)

780

132060

13584.6

228216D

T3

Omaxim (O)

800

146080

70100

118205D

T4

Copper oxychloride (CO)

850

167990

43820

102146D

T5

Apron star (A)

900

-900D

260100

3344

Seed treatment + Seedling treatment +Foliar Spraying

T6

O

12800

382300

3199.16

373192

950.35

T7

A+O+O

12800

296800D

259205D

T8

T5+O

12900

-10560D

313105D

T9

CH

14280

129000D

298715D

T10

CO

17100

144720D

106895D

T11

A+CO+CO

17100

202140D

120888D

T12

T5+CO

17150

-17150D

320854D

4. Conclusion and Recommendation
On the basis of present investigation it may be concluded that seed treatment followed by seedling treatment and foliar spraying of fungicide Omaxim (mancozeb + metalaxyl) can be applied to manage the devastating pepper wilt disease. Nevertheless, it is currently unclear whether the fungicides suppress which type of wilt pathogen, because the disease has been observed to be caused by more than four fungal pathogens. Therefore, identification of specific causal pathogen and designing integrated management options need to be done for management of pepper wilt complex diseases in the study area.
Table 3. Effect of fungicides and application methods on wilt incidence, yield and economic analysis of pepper at Dera (On farm).

Tr. No.

Treatments

WI (%)

DR (%)

DPY (q/ha)

TVC (ETB/ha)

NB (ETB/ha)

MRR (%)

Seed Treatment + Seedling treatment

T1

Check

62.5

-

1.69

0

30420

T2

Sinoko (CH)

25

37.5

20.48

780

367860

43261.5

T3

Omaxim (O)

20.28

42.22

26.36

800

473680

529100

T4

Copper oxychloride (CO)

15.28

47.22

41.66

850

749030

550700

T5

Apron star (A)

57.94

4.56

0

900

-900D

Seed treatment + Seedling treatment +Foliar Spraying

T6

Omaxim

11.11

51.39

48.46

12800

859480

928.15

T7

A+O+O

25

37.5

40.07

12800

708460D

T8

T5+ Omaxim

65.28

-2.78

16.8

12900

289500D

T9

Sinoko

11.95

50.55

35.1

14280

617520D

T10

CO

12.5

50

36.22

17100

634860D

T11

A+CO+CO

11.11

51.39

40.95

17100

720000D

T12

T5+ CO

93.05

-30.55

0.76

17150

-3470D

CV (%)

26.26

-

39.14

LSD (%)

15.157

-

17.15

Abbreviations

TVC

Total Variable Cost

NB

Net Benefit

MRR

Marginal Rate of Return

D

Dominated Treatment

LSD

Least Significant Difference

CV

Coefficient of Variation

WI

Wilt Incidence

DR

Disease Reduction

DPY

Dray Pod Yield

TVC

Total Variable Cost

GPY

Green Pod Yield

Acknowledgments
We would like to thank the Ethiopian Institute of Agricultural Research (EIAR) for the financial and logistic support.
Conflicts of Interest
The authors declare no conflicts of interest.
References
[1] Berke, T. (2002). The VB KL Asian Vegetable Research Development Canter Pepper Project.
[2] Beyene T. and David Phillips, 2007. Ensuring Small Scale Producers in Ethiopia to Achieve Sustainable and Fair Access to Pepper Market. Uganda Journal of Agriculture, 3(2): 113-119.
[3] Fekadu, M., & Dandena, G. (2006). Review of the status of vegetable crops production and marketing in Ethiopia. Uganda Journal of agricultural sciences, 12(2), 26-30.
[4] Adina, G., Yalew, D., & Berhan, M. (2021). Survey on Major Diseases of Vegetable and Tuber Crops in South Gondar Zone, Ethiopia. Results of Plant Protection Research. Proceedings of the Completed Plant Protection Research Activities, 106-111.
[5] Shali, S., 2000. Studies on chilli wilt in Jammu. Sc Thesis submitted to Sher-e-Kashmir University of Agriculture Science and Technology Jammu, p. 45. Journal of Phytopathology, 27(1), pp. 61-68.
[6] Najar, A. G., 2001. Cause and management of chilli wilt in Kashmir (Doctoral dissertation, PhD thesis, SK University of Agricultural Science and Technology of Kashmir, Shalimar, Srinagar Jammu and Kashmir, India).
[7] Rather, T. R., Razdan, V. K., Tewari, A. K., Shanaz, E., Bhat, Z. A., Hassan, M. G. and Wani, T. A., 2012. Integrated management of wilt complex disease in bell pepper (Capsicum annuum L.). Journal of agricultural science, 4(7), pp. 141-147.
[8] Mekonnen Assefa, Woubit Dawit, Alemu Lencho and Tariku Hunduma, 2015. Assessment of Wilt Intensity and Identification of Causal Fungal and Bacterial Pathogens on Hot Pepper (Capsicum Annuum L.) in Bako Tibbe and Nonno Districts of West Shewa Zone, Ethiopia, International Journal of Phytopathology, ISSN: 2305-106X (Online), 2306-1650.
[9] Babadoost, M. and Islam, S. Z., 2003. Fungicide seed treatment effects on seedling damping-off of pumpkin caused by Phytophthora capsici. Plant disease, 87(1), pp. 63-68.
[10] Dubey, S. C., & Patel, B. (2001). Determination of tolerance in Thanatephorus cucumeris, Trichoderma viride, Gliocladium virens and Rhizobium sp. to fungicides. Indian Phytopathology, 54(1), 98-101.
[11] Cimmyt, Mexico, and Mexico Cimmyt. From agronomic data to farmer recommendations: An economics training manual. CIMMYT, 1988.
[12] Stephen, K. and Nicky, M., 2007. In-Service training on partial budgeting techniques. Mount Makulu, Lusaka, Zambia.
[13] Khan, I. H. and Javaid, A., 2015. Chemical control of collar rot disease of chickpea. Pakistan.
Cite This Article
  • APA Style

    Getinet, A., Yalew, D. (2024). Effect of Fungicides and Application Methods for the Management of Pepper Wilt Complex Diseases in Ethiopia. Advances in Bioscience and Bioengineering, 12(4), 93-97. https://doi.org/10.11648/j.abb.20241204.13

    Copy | Download

    ACS Style

    Getinet, A.; Yalew, D. Effect of Fungicides and Application Methods for the Management of Pepper Wilt Complex Diseases in Ethiopia. Adv. BioSci. Bioeng. 2024, 12(4), 93-97. doi: 10.11648/j.abb.20241204.13

    Copy | Download

    AMA Style

    Getinet A, Yalew D. Effect of Fungicides and Application Methods for the Management of Pepper Wilt Complex Diseases in Ethiopia. Adv BioSci Bioeng. 2024;12(4):93-97. doi: 10.11648/j.abb.20241204.13

    Copy | Download

  • @article{10.11648/j.abb.20241204.13,
      author = {Adina Getinet and Desalegn Yalew},
      title = {Effect of Fungicides and Application Methods for the Management of Pepper Wilt Complex Diseases in Ethiopia
    },
      journal = {Advances in Bioscience and Bioengineering},
      volume = {12},
      number = {4},
      pages = {93-97},
      doi = {10.11648/j.abb.20241204.13},
      url = {https://doi.org/10.11648/j.abb.20241204.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20241204.13},
      abstract = {Pepper Wilt complex disease is one of the devastating soil-borne diseases, due to which 68-70% yield loss is recorded. Effects of different fungicides and application methods on wilt incidence and yield of pepper (Capsicum annuum L.) were tested for two years at Fogera and for one year at Dera districts of South Gondar zone Ethiopia. Effects of fungicides viz. copper oxychloride (Isacope 50WP), mancozeb + metalaxyl (Omaxim), thiamethoxam + metalaxyl + difenoconazole (Apron Star 42WS), copper hydroxide (Sinoko) and application methods viz. seed +seedling treatment, seed + seedling treatment followed by spraying at crown region of the plant on wilt disease incidence and pepper yield were studied at Fogera and Dera districts of South Gondar zone Ethiopia. The fungicides were applied at the rates of 3 kg/ha, 2 kg/ha, 20 g/ha and 2.5 kg/ha for Isacope 50WP, Omaxim, Apron Star 42WS and Sinoko, respectively. The seeds of pepper treated with fungicides and untreated were sown in seedling bed. The 45-day old seedlings were uprooted from the seed bed and subjected to the seedling treatment in different fungicides for 30 minutes and were planted in field. The spraying of the fungicides at crown region of the plant was done at 15 days interval after the initiation of wilt disease. The data on wilt incidence and yield were recorded in each treatment. The price of pepper was assessed from the local market and the total price of the yield obtained from each treatment was computed on hectare basis. Input costs like fungicide and labor were converted into hectare basis according to their frequencies used. Based on the obtained data from the above-mentioned parameters economic analysis was performed. The result of the experiment indicates that, during 2020 year, the highest disease reduction (61.11% and 51.39%) over check and maximum dry pod yield (21.95 q/ha and 48.46 q/ha) was observed with T6 (seed treatment+ seedling treatment +foliar spraying with fungicide Omaxim (mancozeb + metalaxyl) at Fogera and Dera, respectively. Similarly, highest disease reduction (26.66%) and maximum green pod yield was observed in T6 at Fogera during 2021 year. Moreover, economic analysis revealed that T6 (application of seed treatment+ seedling treatment + foliar spraying of fungicide Omaxim within 15 days interval) is effective treatment.
    },
     year = {2024}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Effect of Fungicides and Application Methods for the Management of Pepper Wilt Complex Diseases in Ethiopia
    
    AU  - Adina Getinet
    AU  - Desalegn Yalew
    Y1  - 2024/12/27
    PY  - 2024
    N1  - https://doi.org/10.11648/j.abb.20241204.13
    DO  - 10.11648/j.abb.20241204.13
    T2  - Advances in Bioscience and Bioengineering
    JF  - Advances in Bioscience and Bioengineering
    JO  - Advances in Bioscience and Bioengineering
    SP  - 93
    EP  - 97
    PB  - Science Publishing Group
    SN  - 2330-4162
    UR  - https://doi.org/10.11648/j.abb.20241204.13
    AB  - Pepper Wilt complex disease is one of the devastating soil-borne diseases, due to which 68-70% yield loss is recorded. Effects of different fungicides and application methods on wilt incidence and yield of pepper (Capsicum annuum L.) were tested for two years at Fogera and for one year at Dera districts of South Gondar zone Ethiopia. Effects of fungicides viz. copper oxychloride (Isacope 50WP), mancozeb + metalaxyl (Omaxim), thiamethoxam + metalaxyl + difenoconazole (Apron Star 42WS), copper hydroxide (Sinoko) and application methods viz. seed +seedling treatment, seed + seedling treatment followed by spraying at crown region of the plant on wilt disease incidence and pepper yield were studied at Fogera and Dera districts of South Gondar zone Ethiopia. The fungicides were applied at the rates of 3 kg/ha, 2 kg/ha, 20 g/ha and 2.5 kg/ha for Isacope 50WP, Omaxim, Apron Star 42WS and Sinoko, respectively. The seeds of pepper treated with fungicides and untreated were sown in seedling bed. The 45-day old seedlings were uprooted from the seed bed and subjected to the seedling treatment in different fungicides for 30 minutes and were planted in field. The spraying of the fungicides at crown region of the plant was done at 15 days interval after the initiation of wilt disease. The data on wilt incidence and yield were recorded in each treatment. The price of pepper was assessed from the local market and the total price of the yield obtained from each treatment was computed on hectare basis. Input costs like fungicide and labor were converted into hectare basis according to their frequencies used. Based on the obtained data from the above-mentioned parameters economic analysis was performed. The result of the experiment indicates that, during 2020 year, the highest disease reduction (61.11% and 51.39%) over check and maximum dry pod yield (21.95 q/ha and 48.46 q/ha) was observed with T6 (seed treatment+ seedling treatment +foliar spraying with fungicide Omaxim (mancozeb + metalaxyl) at Fogera and Dera, respectively. Similarly, highest disease reduction (26.66%) and maximum green pod yield was observed in T6 at Fogera during 2021 year. Moreover, economic analysis revealed that T6 (application of seed treatment+ seedling treatment + foliar spraying of fungicide Omaxim within 15 days interval) is effective treatment.
    
    VL  - 12
    IS  - 4
    ER  - 

    Copy | Download

Author Information